| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Gramella forsetii | AACTTGCGCAACGACCTG | TCTGCCCTTCTGCGATTGG | 58.98 | 60.08 | 207 | 132 |
100.00%
|
100.00%
|
| Primer 2 | Gramella forsetii | TTAGCCGTGGAGTTGGTGG | TTGCTCTGTCACCCCAAGG | 59.63 | 59.54 | 299 | 129 |
100.00%
|
100.00%
|
| Primer 3 | Gramella forsetii | GCGCAACCAATGAATGCTG | CCAGCTCTGCTAGTTGTTGC | 58.63 | 59.20 | 295 | 128 |
100.00%
|
100.00%
|
| Primer 4 | Gramella forsetii | CGAACCATTATGTGCGTATGC | ACCATGGTGAAAAGCGCTTC | 58.10 | 59.40 | 276 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Gramella forsetii | CGAACCATTATGTGCGTATGC | TGGTGAAAAGCGCTTCTAGC | 58.10 | 58.84 | 272 | 128 |
100.00%
|
100.00%
|
| Primer 6 | Gramella forsetii | TTTTGGGCTGCTTGGTGC | GCTACTCCAACCTGACGTCC | 59.18 | 60.11 | 203 | 128 |
100.00%
|
100.00%
|
| Primer 7 | Gramella forsetii | AGCGTTTCCGGTAGACACC | AGATCCCAGCCTCTTGCAG | 59.71 | 59.09 | 183 | 121 |
100.00%
|
100.00%
|
| Primer 8 | Gramella forsetii | ACCCTGGAACTCAGTGCAC | TCGTCCGCAGATAGATCATGC | 59.55 | 60.07 | 260 | 121 |
100.00%
|
100.00%
|
| Primer 9 | Gramella forsetii | CCGGAAGAAGGACCAGAAGG | TGGGTCTTTTAGCGTTGCTG | 59.75 | 58.77 | 165 | 118 |
100.00%
|
100.00%
|
| Primer 10 | Gramella forsetii | ACCGATGTTGCCAAGGAGG | TGGGGAGTGCAAGTTCAGC | 60.00 | 60.23 | 205 | 116 |
100.00%
|
100.00%
|